HATRI news
6 June 2021
INFORMATION FROM NCBI :
NATIONAL CENTER FOR BIOTECHNOLOGY INFORMATION
HAS BEEN ACCEPTED WITH CODE MT: MN519731.1
The DNA barcode of Baccaurea ramiflora from Phong Dien, Can Tho city, Vietnam based on matK gene
Nguyen thi Lang
Abstract.
The DNA barcode of Baccaurea ramiflora cultivar from based on matK gene. The Baccaurea ramiflora , used for food fresh and juice drink. Based on morphological characters, there are 23 cultivars of Baccaurea ramiflora. A standard method for plant species identification through DNA barcodes has been recommended to use matK gene. The research aimed to determine similarities in DNA barcode sequences of 23 Baccaurea ramiflora fruit cultivars with its close relative that listed in NCBI system and to recommend the reliable DNA barcode for identification of this cultivar. Polymerase Chain Reaction was employed DNA to amplify matK gene fragments using an available forward primer (5’CGA TCT ATT CAT TCA ATA TTT C 3’) and reverse primer (5’TCT AGC ACA CGA AAGTCG AAG T 3’). The software BLAST, Bioedit, and ClustalX were used to analyze the data. Barcode DNA of Baccaurea ramiflora showed 474 bp nucleotides sequence based on matK gene. An indication of six Baccaurea ramiflora cultivars showed that high similarity 99% with Pandanus conoideus Lam. It can be concluded that matK gene can be used to determine the species level in Phyllanthaceae. Locus : MN519731.1. Please see file
Key words: Baccaurea ramiflora; BLAST, Bioedit, gen MatK
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