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HATRI news
Application marker assisted selection in breeding improvement
For salinity tolerance on rice Orysa sativa. L
Nguyen Trong Phuoc, Nguyen thi Lang, Bui Chi Buu
HATRI (High Agricultural Technology Research Institute, Mekong Delta)
This had published their work on Journal of Agriculture and Rural Development. No:5:3-9. 2021
Abstract
Climate change has had a great impact on rice crops in the Mekong Delta. This paper mainly apply GGT from popualtionfrom BC3F3populations on OM1490/Pokkali carrying tolerance sality through molecular markers application to improve salinity rice varieties. In the OM 1490/ Pokkali population. There is a polymorphic on with two indicators RM3252-S1-1 on chromosome 1 and RM223 on chromosome 8. This result is achieved by using 31 indicators on chromo some 1 with a genetic distance of 1-97cM and 21 molecular indicators on chromosome. 8, the genetic distance from 0-56 cM. The selected individuals must carry a superior co-gene on a chromosome that has 4 individuals with 100% of the genes that overlap with (Pokklai), carrying a f salinity target gene. Selected hybrid individuals must carry dominant contracted genes on the chromosome region with 4 lines of BC3F3-11, BC3F3-40, BC3F3-51, and BC3F3-52 with 100% of the gene pool sterilization with the father individual (Pokkali), carrying the salt target gene thanks to GGT on chromosome and five lines BC3F3-16, BC3F3-18, BC3F3-34, BC3F3-48 and BC3F3-51 on chromosome 8. Selected individuals that carry salty genes and recorded yields have: BC3F3-40, BC3F3-52 and line BC3F3-51. Thus, it provides important references for the cultivation of new varieties of tolerance sality rice.
Keywords: application, genetic breeding, GGT; salinity rice, marker.
Bảng 1: Yield and yield components 8 lines from OM1490/Pokkaliat the 2018-2019 Dong Xuan season
|
No
|
Lines
|
Yield(ton/ha)
|
Spikelet/Panicle
(grain)
|
% unfilling
|
P 1000
(g)
|
Panicle /m2
|
|
1
|
BC3F3-11
|
7,66
|
142
|
14,10
|
26,40
|
390
|
|
2
|
BC3F3-40
|
8,30
|
137
|
12,80
|
28,00
|
363
|
|
3
|
BC3F3-51
|
8,30
|
122
|
9,80
|
26,20
|
338
|
|
4
|
BC3F3-52
|
8,10
|
180
|
11,80
|
28,70
|
357
|
|
5
|
BC3F3-16
|
7,66
|
122
|
8,80
|
26,60
|
374
|
|
6
|
BC3F3-18
|
7,63
|
124
|
26,60
|
26,20
|
357
|
|
7
|
BC3F3-34
|
7,60
|
112
|
13,00
|
24,10
|
360
|
|
8
|
BC3F3-48
|
7,56
|
152
|
14,40
|
23,40
|
390
|
|
9
|
OM4900(Đ/C)
|
7,60
|
124
|
13,00
|
26,15
|
360
|
|
|
LSD 5%
|
0,94
|
32,16
|
8,59
|
|
80
|
|
|
CV%
|
7,80
|
16,60
|
27,50
|
|
13,70
|
