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HATRI news
Application marker assisted selection in breeding improvement
For seed size on peanut (Arachis hypogaea L)
Nguyen thị Lang , Le Minh Khang,Nguyen Thị Hong Loan , Nguyen Van Huu Linh , Nguyen thị Khanh Tran, Le Hoang Phuong
HATRI (High Agricultural Technology Research Institute, Mekong Delta)
This had published their work on Journal of Agriculture and Rural Development. No :18 :P100-106. 2021
Abstract
Seed size and quality are important for breeding and production, thus, a more mechanistic understanding of pod development and seed maturation would benefit the improvement of these traits. During pod development, seed filling plays an important role due to the translocation of organic and inorganic compounds and is an important yield component.The rapid development of peanut marker technology, great progress has been made in understanding the increase seed size in peanut, and a series of functional markers has been developed for seed size gene and breeding new peanut varieties in recent years. This paper mainly apply GGT from popualtion F7 from HATRI 01ĐP/ HATRI 02ĐP. There is a polymorphic on the three seeds size positioning test is Aradu-A07-1148327, Aradu-A07-1316694 and GM 1494; on chromosome A07. According to the GGT map, it is easy to identify the genetic pattern of the hybrids in populations compared to the genomic of father and mother in the generation HATRI 01ĐP/ HATRI 02ĐP in the F7 generation. Adopted by 20SNP and two SSR marker on chromosome number A07, the genetic distance from 0-50.8 cM. The selected individuals must carry a superior co-gene on a chromosome that has 4 individuals with 100% of the genes that large seed size with (HATRI 02ĐP), carrying a large size seed target gene. The selected 4 lines as: line F2-4-10-1-1-14; F2-18-11-26-19-20; (F2 -105-3-1-6-21); F2-215-75-86-35-22). However after evaluated F8 and compared phenotype and genotypic only two lines 20 (F2-18-11-26-19-20); and line 21(F2 -105-3-1-6-21)good for selected size seed. Thus, it provides important references for the cultivation of new varieties of peanut in future. Detail
Keywords: genetic breeding, GGT, seed size, peanut,single-nucleotide polymorphism (SNP) markers,simple sequence repeat (SSR) markers.
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B
Figure 1. PCR products of F 1 from HATRI 01D0P/HATRI02D0P (A) và F2 (B) with SNP marker GM 1494 . Linked with size grain on chromosome A07, with size 220bp( HATRI 01 ĐP ) and 200bp(HATRI 02 ĐP), on gel agarose 3 %.
Notes :P1: HATRI 01 ĐP; P2: HATRI 02ĐP.1-50 from population F2
