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HATRI news
This had published their work on Journal of Agriculture and Rural Development. No :24 :P18-27. 2020
Genetic diversity studies mango (Mangifera indica L.) by simple sequence repeat markers (SSR).
Nguyen ThiLang, Nguyen Thi Khanh Tran, Bui Chi Cong, Bui Chi Hieu , và Bui Chi Buu
High Agricultural Technology Research Institute for Mekong delta
SSR markers researh for have been used to genetic distinctiveness among 30 mango varieties. Out of the 66 primers for SSR 31 primers reproducte a total 1,178 bands of which 130 alen were polymorphic, The average number of fragment amplified by SSR marker across the mango have been 130-400 bp. SSR markers across that from 90-100% polymorphism. Information contents: PIC (0.17 to 0.85),genetice diversity H (= 0.351) and allele / locus - AEP (10,91-11,35 Shannon I (5.312). Band analysied by UPMGA (Unweighted pair group method with arithmetic Mean) showed four major clusters and close relatedress amond 30 varieties. This is maybe significant for designing breeding program towards. Some markers good for efficient breeding such as MiIIHR_i929; MiKVR_m056, Lang Xoai F4-R4 and LangXoaiF2-R2. The analysis of the population structure of the individuals also showed four subpopulations, which supported the generated dendrogram. The findings of this study facilitate improved conservation management of the germplasm and help to find strategies for future breeding activities.
Keywords: Multi-image analysis, SSR markers, mango varieties, primer, genetic parameters.
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A B
Figure 1: Primer Lang Xoài F4-R4 's and MiIIHR_i929 .PCR product on 122 different rice varieties is separated on polyacrylamide gel dyed with silver nitrate
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Figure 2. The genealogy diagram shows a genetic correlation between 30 mango varieties based on the Jaccard similarities index using the SSR marker (UPGMA). A, B, C, and D show that there are 4 main groups, when considering the similar score from 0.47-1.
Table 1. Evaluation of mango traits of 30 varieties growing in Mekong delta , VietNam
|
lines
|
Hight plant (m)
|
Stumps
(M)
|
Canopy
(M)
|
Length leaf (Cm)
|
Wide leaf (Cm)
|
Weight fruit(gram)
|
yield (kg
/plant)
|
°Brix)
|
|
Hòalộc1
|
7.00nop
|
1.00 ijk
|
4.00 i
|
32.00 b
|
0.40 o
|
450 b
|
300 p
|
13.2 h
|
|
HòaLộc2
|
8.90 ab
|
0.70 o
|
3.40 jk
|
25.00 r
|
0.50 g-j
|
400 e
|
345 kl
|
13.8 h
|
|
HòaLộc3
|
8.90 ab
|
0.70 o
|
5.00 ef
|
26.00 p
|
0.56 cde
|
320 i-l
|
356 j
|
14.5 g
|
|
HòaLộc4
|
5.40 st
|
1.20 f
|
5.00 ef
|
27.00 o
|
0.57 bcd
|
321 ijk
|
356 j
|
17.1 d
|
|
HòaLộc5
|
8.50 cd
|
1.20 f
|
5.20 e
|
28.00 l
|
0.60 b
|
312 k-n
|
356 j
|
16.5 e
|
|
CátChu1
|
9.00 a
|
0.90 m
|
5.20 e
|
28.90 h
|
0.50 g-j
|
211 r
|
374 i
|
16.8 e
|
|
CátChu2
|
7.80 ghi
|
0.92 lm
|
5.30 de
|
28.60 h-k
|
0.68 a
|
324 hi
|
458 f
|
12.73l a
|
|
CátChu3
|
7.00 nop
|
0.98 jkl
|
5.30 de
|
32.00 b
|
0.59 bc
|
231 q
|
500 e
|
17.5 d
|
|
CátChu4
|
6.80 pq
|
0.97 jkl
|
4.20 hi
|
32.10 b
|
0.60 b
|
312 k-n
|
523 c
|
16.6 e
|
|
CátChu5
|
8.20 def
|
0.95 klm
|
4. i
|
34.20 a
|
0.50 g-j
|
452 b
|
542 b
|
16.8 e
|
|
ĐàiLoan
|
7.60 g-k
|
0.97 jkl
|
4.10 i
|
28.90 h
|
0.54 def
|
451 b
|
421 g
|
12.4 l
|
|
ĐàiLoan2
|
9.00 a
|
1.10 g
|
4.20 hi
|
29.60 efg
|
0.52 fgh
|
421 c
|
411 h
|
14.1g
|
|
ĐàiLoan3
|
7.30 j-n
|
1.20 f
|
4.60 g
|
29.50 efg
|
0.56 cde
|
421 c
|
423 g
|
12.5 l
|
|
ĐàiLoan4
|
7.60 g-k
|
1.30 e
|
4.50 gh
|
27.00 o
|
0.52 fgh
|
412 d
|
415 gh
|
15.6 f
|
|
ĐàiLoan5
|
6.60 q
|
1.40 d
|
4.50 gh
|
27.40 n
|
0.51 f-i
|
412 d
|
523 c
|
14.4 g
|
|
xoàiTượng
|
7.90 fgh
|
1.60 b
|
4.10 i
|
25.60 q
|
0.53 efg
|
590 a
|
624 a
|
11.4l
|
|
ThanhCa
|
8 efg
|
1.20 f
|
3.00 l
|
27.80 lm
|
0.52 fgh
|
530 a
|
521 c
|
20.7b
|
|
XoàiMũ
|
8 efg
|
1.10 g
|
4.00i
|
29.60 efg
|
0.51 f-i
|
320 i-l
|
415 gh
|
12.4l
|
|
XoàiVoi
|
8.50 cd
|
1.02 hij
|
4.20 hi
|
28.90 h
|
0.60 b
|
300 p
|
251 q
|
11.5 l
|
|
XoàiHòn
|
8.30 cde
|
1.03 hij
|
4.00 i
|
24.80 r
|
0.45 lmn
|
310 mno
|
210 t
|
13.9 h
|
|
XoàiÚc1
|
8.40 cd
|
1.05 ghi
|
4.20 hi
|
29.50 efg
|
0.42 no
|
301 p
|
241 r
|
11.4 l
|
|
XoàiÚc2
|
6.90 opq
|
1.07 gh
|
4.20 hi
|
25.80 pq
|
0.65 a
|
305 nop
|
253 q
|
12.4 l
|
|
Xoài TháiLan Xanh
|
7.40 i-m
|
0.90 m
|
3.00 l
|
28.70 hij
|
0.52 fgh
|
304 nop
|
200 u
|
18.6 c
|
|
Xoài TháiLan
|
8.60 bc
|
0.99 ijk
|
3.00 l
|
27.80 lm
|
0.51 f-i
|
342 g
|
145 v
|
17.5 d
|
|
Xoài Keo Xanh
|
7.50 i-l
|
0.95 klm
|
3.20 jkl
|
25.60 q
|
0.52 fgh
|
341 g
|
124 w
|
21.1 a
|
|
Xoài Keo Xanh2
|
4.60 u
|
0.92 lm
|
3.50 j
|
29.80 cde
|
0.54 def
|
342 g
|
120 w
|
20.4 b
|
|
Ấn Độ
|
8.50 cd
|
0.80 n
|
3.10 kl
|
28.50 ijk
|
0.52 fgh
|
324 hi
|
236 rs
|
13.2 h
|
|
Philipine
|
7.60 g-k
|
0.98 jkl
|
4.00 i
|
28.80 hi
|
0.57 bcd
|
331 h
|
356 j
|
17.4d
|
|
Nha Trang
|
7.50 i-l
|
0.79 n
|
6.00 a
|
29.50 efg
|
0.54 def
|
324 hi
|
562 a
|
12.9 l
|
|
KeoBNúi
|
7.40 i-m
|
1.20 f
|
5.20 e
|
27.80 lm
|
0.45 lmn
|
324 hi
|
510 d
|
18.5 c
|
