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This had published their work on Journal of Agriculture and Rural Development. No :24 :P18-27. 2020
28/12/2020 INTERNAL NEWS 29
 
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This had published their work on Journal of Agriculture and Rural Development.  No :24 :P18-27.  2020
 
Genetic diversity studies mango (Mangifera indica L.) by simple sequence repeat markers (SSR).
 
Nguyen ThiLang, Nguyen Thi Khanh Tran, Bui Chi Cong, Bui Chi Hieu , và Bui Chi Buu
 
High Agricultural Technology Research Institute for Mekong delta
 
SSR markers researh for have been used to genetic distinctiveness among 30 mango varieties. Out of the 66 primers for SSR 31 primers reproducte a total 1,178 bands of which 130 alen were polymorphic, The average number of fragment amplified by SSR marker across the mango have been 130-400 bp. SSR markers across that from 90-100% polymorphism. Information contents: PIC (0.17 to 0.85),genetice diversity H­ (= 0.351) and allele / locus - AEP (10,91-11,35 Shannon I (5.312). Band analysied by UPMGA (Unweighted pair group method with arithmetic Mean) showed four major clusters and close relatedress amond 30 varieties. This is maybe significant for designing breeding program towards. Some markers good for efficient breeding such as MiIIHR_i929; MiKVR_m056, Lang Xoai F4-R4 and LangXoaiF2-R2. The analysis of the population structure of the individuals also showed four subpopulations,  which supported the generated dendrogram. The findings of this study facilitate improved conservation management of the germplasm and help to find strategies for future breeding activities.
Keywords: Multi-image analysis, SSR markers, mango varieties, primer, genetic parameters.
 
 
12

A                                                                                                             B
Figure 1: Primer Lang Xoài F4-R4 's and MiIIHR_i929 .PCR product on 122 different rice varieties is separated on polyacrylamide gel dyed with silver nitrate
 
 
Figure 2. The genealogy diagram shows a genetic correlation between  30 mango varieties based on the Jaccard similarities index using the SSR marker (UPGMA). A, B, C, and D show that there are 4 main groups, when considering the similar score from 0.47-1.
 
Table 1.  Evaluation of  mango  traits of 30 varieties growing in Mekong delta , VietNam
 
lines
Hight plant (m)
Stumps
(M)
Canopy
(M)
 Length leaf (Cm)
Wide leaf (Cm)
  Weight  fruit(gram)
 yield (kg
 
/plant)
°Brix)
Hòalộc1
7.00nop
1.00 ijk
4.00 i
32.00 b
0.40 o
450 b
300 p
13.2 h
HòaLộc2
8.90 ab
0.70 o
3.40 jk
25.00 r
0.50 g-j
400 e
345 kl
13.8 h
HòaLộc3
8.90 ab
0.70 o
5.00 ef
26.00 p
0.56 cde
320 i-l
356 j
14.5 g
HòaLộc4
5.40 st
1.20 f
5.00 ef
27.00 o
0.57 bcd
321 ijk
356 j
17.1 d
HòaLộc5
8.50 cd
1.20 f
5.20 e
28.00 l
0.60 b
312 k-n
356 j
16.5 e
CátChu1
9.00 a
0.90 m
5.20 e
28.90 h
0.50 g-j
211 r
374 i
16.8 e
CátChu2
7.80 ghi
0.92 lm
5.30 de
28.60 h-k
0.68 a
324 hi
458 f
12.73l a
CátChu3
7.00 nop
0.98 jkl
5.30 de
32.00 b
0.59 bc
231 q
500 e
17.5 d
CátChu4
6.80 pq
0.97 jkl
4.20 hi
32.10 b
0.60 b
312 k-n
523 c
16.6 e
CátChu5
8.20 def
0.95 klm
4. i
34.20 a
0.50 g-j
452 b
542 b
16.8 e
ĐàiLoan
7.60 g-k
0.97 jkl
4.10 i
28.90 h
0.54 def
451 b
421 g
12.4 l
ĐàiLoan2
9.00 a
1.10 g
4.20 hi
29.60 efg
0.52 fgh
421 c
411 h
14.1g
ĐàiLoan3
7.30 j-n
1.20 f
4.60 g
29.50 efg
0.56 cde
421 c
423 g
12.5 l
ĐàiLoan4
7.60 g-k
1.30 e
4.50 gh
27.00 o
0.52 fgh
412 d
415 gh
15.6 f
ĐàiLoan5
6.60 q
1.40 d
4.50 gh
27.40 n
0.51 f-i
412 d
523 c
14.4 g
xoàiTượng
7.90 fgh
1.60 b
4.10 i
25.60 q
0.53 efg
590 a
624 a
11.4l
ThanhCa
8 efg
1.20 f
3.00 l
27.80 lm
0.52 fgh
530 a
521 c
20.7b
XoàiMũ
8 efg
1.10 g
4.00i
29.60 efg
0.51 f-i
320 i-l
415 gh
12.4l
XoàiVoi
8.50 cd
1.02 hij
4.20 hi
28.90 h
0.60 b
300 p
251 q
11.5 l
XoàiHòn
8.30 cde
1.03 hij
4.00 i
24.80 r
0.45 lmn
310 mno
210 t
13.9 h
XoàiÚc1
8.40 cd
1.05 ghi
4.20 hi
29.50 efg
0.42 no
301 p
241 r
11.4 l
XoàiÚc2
6.90 opq
1.07 gh
4.20 hi
25.80 pq
0.65 a
305 nop
253 q
12.4 l
Xoài TháiLan Xanh
7.40 i-m
0.90 m
3.00 l
28.70 hij
0.52 fgh
304 nop
200 u
18.6 c
Xoài TháiLan
8.60 bc
0.99 ijk
3.00 l
27.80 lm
0.51 f-i
342 g
145 v
17.5 d
Xoài Keo Xanh
7.50 i-l
0.95 klm
3.20 jkl
25.60 q
0.52 fgh
341 g
124 w
21.1 a
Xoài Keo Xanh2
4.60 u
0.92 lm
3.50 j
29.80 cde
0.54 def
342 g
120 w
20.4 b
Ấn Độ
8.50 cd
0.80 n
3.10 kl
28.50 ijk
0.52 fgh
324 hi
236 rs
13.2 h
Philipine
7.60 g-k
0.98 jkl
4.00 i
28.80 hi
0.57 bcd
331 h
356 j
17.4d
Nha Trang
7.50 i-l
0.79 n
6.00 a
29.50 efg
0.54 def
324 hi
562 a
12.9 l
KeoBNúi
7.40 i-m
1.20 f
5.20 e
27.80 lm
0.45 lmn
324 hi
510 d
18.5 c

 
 
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