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HATRI ANNOUNCEMENT ON
RESEARCH ARTICLE
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SABRAO Journal
of Breeding and Genetics
52 (3) P326-340, 2020
BREEDING RICE (Oryza sativa. L) FOR SALT TOLERANCE AND
GRAIN QUALITY TRAITS
N.T. LANG1*, N.T. PHUOC1, B.H. THUAN2and B.C. BUU1
1High Agricultural Technology Research Institute for Mekong delta(HATRI), Vietnam
2Department of Biology, Mekong University, Vietnam
*Corresponding author email: ntlang.prof@gmail.com
Email addresses of coauthors: trongphuoc33@gmail.com, buihuuthuan@mku.edu.vn, buichibuu@gmail.com
SUMMARY
The development of rice cultivars with salinity tolerance, high yield, and good quality is a very essential demand that has been raised to provide poor farmers in the lowland conditions of the coastlines of the Mekong Delta, Vietnam. This study aimed to develop rice cultivars that were tolerant of salinity at the seedling and reproductive stages via molecular marker, single crossing, and backcrossing breeding methods. The salt tolerance of F8and BC3F4 progenies was assessed under salt stress field conditions to select promising lines for improving production by farmers. Salt tolerance alleles on chromosomes one and eight were detected by combining traditional breeding by backcross and generation selection with SSR markers. L1 (OM10252/Pokkali); L5 (OMCS2012/Pokklai); L21 and L22 (OM10252/Pokkali//OM10252); and L24 and L25 (OM8902/Pokkali//OM8902) were identified as high-yielding genotypes with good salinity tolerance. However, many remaining lines failed due to their extended growth duration, high unfilled grain percentage, and poor grain quality. Four lines, namely, viz. L1, L5, L24, and L25, exhibited good agronomic characters, such as low amylose content and salt stress tolerance. The typical tolerant genotype Pokkali was successfully exploited into high-yielding rice cultivars via breeding programs. This is a good opportunity to improve salt tolerance rice cultivars via marker-assisted selection with RM223, RM8094, HATRI02, RM3412, RM493, and RM3252.
Keywords: Chromosome,high-yielding, grain quality, salt, SSR markers
Grain Quality of 14 lines from dry season 2018-2019 at Ba Tri (Ben Tre Province).
|
Lines
|
Brown rice
(%)
|
White
rice
(%)
|
Head
rice
(%)
|
Length (mm)
|
L/W
|
Gelatini-zation tempera-ture (GT) (Score)
|
Chakiness (% score 9)
|
%
Amylose
|
|
L1
|
79.51 ab
|
74.84 def
|
29.33 g
|
7.32 b
|
3.44 ab
|
7.12 a
|
17.67 a
|
19.35 f
|
|
L2
|
78.34 ab
|
75.39 cde
|
41.73 cd
|
7.06 de
|
3.27 cde
|
2.67 def
|
6.67 ef
|
24.63 de
|
|
L5
|
77.7 ab
|
74.62 def
|
41.90 cd
|
6.86 g
|
3.38 abc
|
6.00 b
|
4.00 f
|
19.12 f
|
|
L16
|
79.38 ab
|
76.72 bc
|
39.84 de
|
7.19 c
|
3.34 abcd
|
3.33 cd
|
8.33 cde
|
24.98 cde
|
|
L17
|
79.32 ab
|
74.06 ef
|
39.47 e
|
6.93 fg
|
3.46 a
|
7.00 a
|
7.67 de
|
25.67 bc
|
|
L21
|
79.38 ab
|
77.52 ab
|
44.15 b
|
7.30 b
|
3.34 abcd
|
3.0 c
|
10.00 cd
|
24.63 de
|
|
L22
|
80.04 a
|
77.65 ab
|
41.67 cd
|
7.09 cd
|
3.33 bcd
|
2.33 ef
|
13.00 b
|
24.98 cde
|
|
L24
|
78.04 ab
|
73.72 f
|
43.19 bc
|
7.05 def
|
3.32 cd
|
6.00 b
|
7.33 de
|
20.01 e
|
|
L25
|
77.63ab
|
75.42cde
|
47.38a
|
6.95efg
|
3.26de
|
6.00b
|
6.33ef
|
19.28f
|
|
L27
|
79.86 ab
|
77.69 ab
|
40.55 de
|
7.13 cd
|
3.20 e
|
6.00 b
|
11.33 bc
|
20.00 e
|
|
Checks
|
|
|
|
|
|
|
|
|
|
OM 8012
|
79.23 ab
|
75.73 cd
|
32.77 f
|
7.62 a
|
3.38 abc
|
5.33 c
|
8.33 cde
|
22.15 e
|
|
OM 10252
|
78.54 ab
|
76.49 bc
|
48.40 a
|
6.87 g
|
3.20 e
|
6.00 b
|
8.33 cde
|
20.27 e
|
|
OM CS2012
|
76.50 b
|
78.94 a
|
39.37 e
|
7.16 cd
|
3.37 abcd
|
6.67 b
|
9.33 cde
|
20.98 e
|
|
Pokkali
|
77.6 ab
|
74.61 def
|
42.90 cd
|
6.86 g
|
3.38 abc
|
2.00 e
|
4.00 f
|
26.19 a
|
|
CV (%)
|
2.20
|
1.30
|
2.80
|
0.90
|
1.80
|
10.10
|
18.40
|
1.90
|
HATRI 02
.png)
RM223
.png)
RM493
.png)
RM3252
.png)
RM3412
.png)
RM8094
.png)
Figure 1.PCR products of the line at HATRI 02 (A), RM223 (B), RM493 (C), RM3252 (D), RM3412 (E), and RM8094 (F). The gene associated with resistance to salinity is located at the positions of chromosomes 1 and 8 on 3% agarose gel.
