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Application marker assisted selection in breeding improvement for fragrant gene on rice Orysa sativa. L
13/05/2020 INTERNAL NEWS 165
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Application marker assisted selection in breeding improvement
for fragrant gene on rice Orysa sativa. L
 
Ha Ngoc Hien, Nguyen thi Khanh Tran, Nguyen Thi Lang, Bui Chi Buu
 HATRI (High Agricultural Technology Research Institute, Mekong Delta)
This had published their work on Journal of Agriculture and Rural Development.  No :9 :P3-9.  2020
 
Abstract
  The rapid development of rice marker technology, great progress has been made in understanding the fragrant gene in rice for export, and a series of functional markers has been developed for screening the aroma gene and breeding new rice varieties in recent years. This paper mainly apply GGT from popualtion F7 from OMCS2000/KhaoDawmali 105, of the fragrant gene, and the application of aroma gene functional markers in the genetic improvement and breeding of aromatic rice.
 
  Populations OMCS2000/KhaoDawMali 105 are noted as recessive geneson populations F2 and F3. There is a polymorphic on the two only aroma positioning test is RM223 and RM 3459 on chromosome 8. According to the GGT map, it is easy to identify the genetic pattern of the hybrids in populations compared to the genomic  offather and mother in the generation OMCS2000/KhaoDawMali 105 in the F 7 generation. Adopted by 21 molecular SSR on chromosome number 8, the genetic distance from 0-56 cM. The selected individuals must carry a superior co-gene on a chromosome that has 4 individuals with 100% of the genes that coincidize with (Khao Daw Mali 105), carrying a fragrant target gene. The selected lines as: line 19 (F2-4-6-16-1-7-19); line 20 (F2-17-1-16-19-8-20); line 29 (F2-18-3-1-8-106-29); line 31 (F2-31-7-8-38-10-31). Thus, it provides important references for the cultivation of new varieties of aromatic rice.
 
Keywordsapplication, genetic breeding, GGT; fragrant rice, marker.
 
Figure 1: The F7 generation genetic diversity on the hybrid population OMCS2000/KhaoDawmali 105 on chromosome No. 8.
Note: Blue color: Genomics genotype (KhawDaw Mali 105), Red: Mother Tree Type (OMCS2000), Gray: heterosexual genotype, green frame: Marked selected individuals, 1-50: The individuals of the OMCS2000/KhaoDawmali populations 105.
 
 Table: Yield and components yield of goods lines from OMCS2000/ KhaoDawmali 105 
 
No.
Lines
 Length panicle
 Spikelet / panicle
% unfilling
 Grain weight 1000
Yield (g)
cm
hạt
%
g
hilling
1m2
1
19 (F2-4-6-16-1-7-19)  
25.35 ab
105.50 a
22.34 b
27.32 a
18.31 abc
604.10 abc
2
20 ( F2-17-1-16-19-8-20)
25.84 a
85.70 ab
27.42 ab
27.43 a
13.33 c
439.96 c.
3
29 (F2 -18-3-1-8-106-29)
24.08 bc
88.40 ab
24.23 ab
27.52 a
22.43 a
740.32 a
4
31(F2-31-7-8-38-10-31)
24.57 abc
87.10 ab
24.95 ab
27.03 a
16.22 bc
535.13 bc
5
4 (F2-5-100-8-19-4-4)
23.89 c
81.50 ab
25.53 ab
27.63 a
16.75 bc
552.82 bc
6
39 (F2 – 6-100-4-1-8-10-39)
23.64 c
74.10 b
26.25 ab
26.22 a
15.63 bc
515.63 bc
7
35 (F2-10-8-6-7-18-35)
23.73 c
71.60 b
27.70 ab
27.43 a
15.10 bc
498.27 bc
8
43 (F2-14-16-100-1-7-43)
24.64 abc
89.90 ab
24.96 ab
26.91 a
18.89 abc
623.21 abc
9
34 ( F2-1-100-8-10-6-34) 
23.75 c
92.40 ab
20.86 b
27,51 a
19.26 ab
635.45 ab
10
KHaoDawMali 105 ( đối chứng)
24.51 abc
73.30 b
22.24 a
28.83 a
14.47 bc
477.51 bc
11
 OMCS2000( đối chứng )
24.02 bc
99.40 a
22.87 ab
26.02 a
18.64 abc
615.02 abc
Mean
24.37
84.45
25.4
27.26
17.18
567.04
CV (%)
5.8
25.3
25.6
8.8
31.6
31.6
 
 
 
 
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